ABSTRACT
Objective: to determine the expression of cyclin D1 and PTEN [phosphatase and tensin homolog] in endometrial hyperplasias and neoplasias
Study Design: Analytical study
Place and Duration of Study: the study was conducted at BMSI, JPMC, Karachi, from January 2008 to December 2012
Methodology: analysis of endometrial samples, comprising of hysterectomies and curettage, was carried out. Immunohistochemical staining was done for PTEN and cyclin D1 expression
Results: fifty-three endometrial samples including 23 endometrial carcinomas, 6 complex hyperplasias with atypia, 14 complex hyperplasias without atypia, 6 simple hyperplasias without atypia and 4 normal proliferative endometrium were analyzed. Fifty-two percent [12 out of 23] and 48% [11 out of 23] cases of endometrial carcinomas showed complete loss of PTEN expression and cyclin D1 over expression, respectively. Five [5 out of 6] cases of complex hyperplasias with atypia and 64.28% [9 out of 14] cases of complex hyperplasia without atypia showed complete loss of or diminished expression of PTEN whereas 66.66% [4 out of 6] cases of endometrial hyperplasia with atypia and 50% [7 out of 14] cases of endometrial hyperplasia without atypia showed cyclin D1 overexpression [p < 0.001]
Conclusion: loss of PTEN, expression and cyclin D1 overexpression was seen in a significant number of well differentiated endometrial adenocarcinomas and complex hyperplasias with atypia, suggesting both as an early event in endometrial carcinogenesis
ABSTRACT
To observe the differential expression of p63 in hydropic and molar gestation. Cross-sectional analytical study. Department of Pathology, Basic Medical Sciences Institute, Jinnah Postgraduate and Medical Centre, Karachi, from January 2006 to June 2013. Ninety placental biopsies including 30 cases each of hydropic abortions, partial hydatidiform mole and complete hydatidiform mole were analyzed for morphological features and results of immunohistochemical staining. Results were described as frequency. Significance was determined using test of proportions with significance at p < 0.05. Out of 30 cases of hydropic abortion, 6 were negative, 15 were weak, 4 were moderate and 5 showed strong degree of intensity for p63. Out of 30 cases of partial hydatidiform mole, 3 were negative, 2 showed weak, 4 showed moderate and 21 cases showed strong degree of intensity for p63. All 30 cases of complete hydatidiform mole strongly stained for p63. The intensity of staining of p63 was stronger in cases of molar pregnancy as compared to hydropic abortion. There was loss of p63 expression in cytotrophoblastic cells in all abortions. In limited resources settings, where facilities for PCR/FISH and DNA ploidy analysis is not available, the authors advocate p63 in routine clinical practice to provide the most refined diagnosis of hydatidiform moles